Journal: bioRxiv
Article Title: Biomimetic miR-133a inhibitor activated scaffolds optimised for spinal cord repair promote neurite outgrowth and angiogenesis via neuronal cytoskeletal remodelling
doi: 10.64898/2026.04.22.719922
Figure Lengend Snippet: A) Venn diagram of predicted miR-133a targets identified across three public miRNA–mRNA interaction databases (TargetScan, miRDB and StarBase), showing the shared gene set used for downstream enrichment analysis. B) Gene Ontology (GO) enrichment analysis of common predicted miR-133a targets, highlighting over-represented neuronal and cytoskeletal categories. C) KEGG pathway enrichment analysis of the same gene set, demonstrating enrichment of pathways linked to synaptic vesicle cycling, regulation of the actin cytoskeleton and adherens junctions. D-E) Size distribution spectra of fresh and lyophilised RALA/miR-133a-i nanoparticles formulated at an N:P 8 ratio. Insert summarises z-average, polydispersity index (PDI), mean count rate and zeta potential +/- SEM. F) Mean hydrodynamic size and PDI of fresh and lyophilised RALA/miR-133a-i nanoparticles at N:P ratio of 8. G) Ion exchange chromatography results showing absorbance spectra of RALA/ miR-133a-i nanoparticles, water and unencapsulated miRNA following chromatography through an anionic Sephadex resin. H) Encapsulation efficiency of RALA/miR-133a-i nanoparticles measured by quantifying free miRNA present in solution. I) TEM image showing RALA/miR-133a-i nanoparticles. J) Loading efficiency of >96% of nanoparticles in hyaluronic acid scaffolds was detected by the Ribogreen Assay. Nanoparticle release kinetics measured in ng and percentage change for short-term release (K-L) and long-term release periods (M-N) demonstrated an initial burst release followed by a slower, consistent release.
Article Snippet: Libraries were prepared and sequenced by Novogene (UK) using poly(A)-enriched mRNA libraries and a NovaSeq X Plus platform (paired-end 150 bp; N=4 untreated, N=5 miR-133a-i-activated).
Techniques: Zeta Potential Analyzer, Ion Exchange Chromatography, Chromatography, Encapsulation
